Cytogenetic analysis of myocardial progenitor cells of rats at early passages

УДК 576. 316:611. 018. 63:636. 96

O. Kovpak

Introduction. Despite significant advances in modern medicine treatment of myocardial infarction neither conservative nor operative methods do not give the desired result. A promising method of treatment of heart pathologies is the using of cellular technology.
Progenitor stem cells are poorly expressed complexes of major histocompatibility antigens which reduce likelihood after transplantation complications. In order to use myocardial progenitor cells for clinical purposes the significant quantity of cells is needed, that can be achieved only by long-standing in vitro cultivation. Literature data analysis doesn’t give an unambiguous answer regarding genetic stability of myocardial progenitor cells during their in vitro cultivation, which necessitated further research.
Goal of the work. Performing of cytogenetic analysis of myocardial progenitor cells of rats at early passages.
Materials and methods.Myocardial progenitor cells of rats of the first to the sixth passages were used in this research. The cytogenetic analysis was performed on 30 metaphase plates of rat’sstem cells from every passage. Slides were obtained through modification of standard cytogenetic method. In the course of the research we considered: quantitative abnormalities of chromosomes – aneuploidy, polyploidy. The same preparations were used to calculate the quantity of binuclear cells, cells with micronuclei, mitotic index, apoptotic cells (frequency was calculated for 500 cells (%)).
Results of research and discussion. Presented the results of cytogenetic analysis of myocardial progenitor cells of rats during in vitro culture. We found alterations in genetic apparatus of cells, that occurred in the form of aneuploidy, polyploidy as well as micronuclei, the amount of which varied depending on the passage. However, the variability of karyotype of the mentioned cells didn’t exceed self-existing level of mutations, specific to this animal species.

Key words: cytogenetic analysis, micronucleus test, progenitor cells, myocardium.

Cytogenetic analysis of myocardial progenitor cells of rats at early passages.

Issue 2(89), Part 1, 2016