Genetic polymorphism of the red white belted breed pigs based on microsatellite markers

UDC 636.4.082.25:575.22

S. Lugovoy, PhD (Agr.), Ass. Professor
S. Kramarenko, DSc (Biol.), Ass. Professor
S. Galimov, PhD (Agr.), Ass. Professor
Mykolayiv National Agrarian University

The aim of this study was to analyze the genetic variability and population structure of the Red White Belted breed pigs. Twelve microsatellite loci were selected and belong to the list of microsatellite markers recommended by FAO/ISAG. The number of observed alleles (Na) detected ranged from 4 to 8, with an overall mean of 6.67 and a total of 80 alleles were observed at these loci. The overall means for observed (Ho) and expected (He) heterozygosities were 0.649 and 0.693, respectively. The population effective size (Ne) estimate based on LD-method is 59.2 (95% CI: 36-129) individuals. This population has not undergone any recent and/or sudden reduction in the effective population size and remained at mutation-drift equilibrium.

Key words: genetic polymorphism, microsatellite loci, pigs, the Red White Belted breed.

Genetic polymorphism of the red white belted breed pigs based on microsatellite markers.

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Issue №1 (93), 2017

Assessment of the genetic structure of different families of Duroc pigs at loci of microsatellites

UDC 636.082

S. Lugovoy, S. Kish.

To avoid future problems in agriculture and food industry, there is a clear need for significant efforts to conserve genetic resources. Development of effective mechanism for achieving this goal requires systematic monitoring of genetic processes in populations. In pig breeding as a tool for assessing biodiversity commercial, local and rare breeds are often use microsatellite polymorphism loci. In Ukraine is now are breeding 11 domestic and foreign breeds of pigs. In particular, created a new interbreed type of Ukrainian selection “Stepoviy”.
The aim of the research was to establish the genetic characteristics of various families of Duroc pigs on microsatellite DNA polymorphism loci. For the study we used data from the genetic polymorphism of 12 loci microsatellites of seven families of pigs Duroc, which are bred in a breeding plant PJSC “Pedigree farm “Stepoviy” Zaporozhye region. In the context of families, the number of animals was: Rosynka – 16 animals, Liliya – 7 animals, Muzyla – 11 animals, Romashka – 14 animals, Avgusta – 12 animals, Vishnya – 8 animals, Gastela – 4 animals. The material for DNA extraction were tissue samples (ear notching) of pigs. Preservative were performed as 96% ethanol. Laboratory studies were performed at the Center of biotechnology and molecular diagnostics of the All-Russian Research Institute of animal breeding of the RAAS.
It is established that different families of Duroc pigs are characterized by some specific features of the genetic profiles of genetic loci microsatellite DNA profiles. Evidence of this is the presence of private alleles, as well as differing degrees of heterozygosity. The most genetically differentiated among themselves have families of Gastelas and Liliyas, and most similar – Romashkas and Rosynkas. Peculiarities of the allelic profiles of each of the families can be the basis for the definition of marker alleles that could be used in further in-depth breeding, and genetic identification and confirmation of animal origin.

Key words: pig breeding, breeding, breeding records, information systems.

S. Lugovoi, S. Kish. Assessment of the genetic structure of different families of Duroc pigs at loci of microsatellites.


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С. И. Луговой, С. В. Киш. Оценка генетической структуры различных семейств свиней породы дюрок по локусам микросателлитов ДНК.

В статье приведены результаты оценки генетической структуры различных семейств свиней породы дюрок на основе полиморфизма локусов микросателлитов ДНК. Установлено, что разные семейства характеризуются определенной специфичностью аллельных профилей, которая может быть использована при дальнейшей углубленной племенной работе, а также для генетической идентификации и подтверждения происхождения животных.

С. І. Луговий, С. В. Кіш. Оцінка генетичної структури різних родин свиней породи дюрок за локусами мікросателітів ДНК.

У статті наведено результати оцінки генетичної структури різних родин свиней породи дюрок на основі поліморфізму локусів мікросателітів ДНК. Встановлено, що різні родини характеризуються певною специфічністю алельних профілів, яка може бути використана при подальшій поглибленій племінній роботі, а також для генетичної ідентифікації та підтвердження походження тварин.

Technology of production of pork in terms of LLC “Tavriyski svyni”

УДК 636.4.082

V. Topiha
V. Lykhach
S. Lugovoy
P. Shebanin

The creation of meat balance in the country is primarily dependent on the increase in the production of all kinds of meat, including pork, which in beef balance should occupy more than 35%. Therefore, to increase pork production, improving quality and output of the pig industry on a global level, the maximum potential use pigs for human needs, it is necessary to efficiently use tribal resources of pigs in Ukraine and in the world, enhance forage and implement cutting-edge technologies and scientific achievements. Currently breeding in the pig industry of Ukraine is characterized by the trend of imports of farm animals beef cattle productivity of foreign origin. Proven a number of studies that our local genotypes in creating optimal conditions of feeding and maintenance, performance is not inferior to foreign ones, and in terms of sustainability, adaptation to the conditions of feeding and maintenance, typical of most farms, as well as the quality of the products is significantly higher. This necessitates a more thorough study of the productive qualities of domestic pigs genotypes and development of their breeding programs. In Ukraine the third largest population is Ukrainian meat breed, but the number of sows and boars of this breed for the period from 1993 to 2013 decreased by 45.8 and 62.3%, respectively. Therefore, significant has been the preservation, dissemination and improvement of the aforementioned pig genotype. Given the relevance of the use of swine meat genotypes to increase pork production was supplied for the purpose, in the article, to provide an analysis of breeding work with pigs breeds Ukrainian meat (Askania type) and large white foreign selection. The study was conducted in the conditions of breeding plant breeding pigs Askania type of Ukrainian meat breed and pedigree reproducer breeding pigs of large white breed a Limited liability company (LLC) “Tavriyski svyni” Skadovsk district, Kherson region. Determined that today the industry in the economy presents a plant with breeding pigs Askania type of Ukrainian meat breed – a 125 main sows and breeding facility for the breeding of pigs of large white breed – 93 major sows. The introduction of modern technology has given the opportunity to get quite good results productivity of animals. It is established that in the breeding factory LLC “Tavriyski svyni” are bred and grown such breeds of pigs which are unsurpassed reproducible, fattening and meat qualities in terms of the South of Ukraine. Found that the industry in agriculture has become profitable and competitive among the farms of the region and the country. Every year, it is grown and available for the implementation of 600 head of young pigs Ukrainian meat breed and 400 head of young pigs of large white breed, which is 90% meets the requirements of class elite. The farm has the possibility of implementing two-breed pigs such combinations: Ukrainian meat × Landrace and large white × Landrace.

technology, pigs, breed, productive, quality appraisal.


Analysis of Effectiveness of Using Selection Indices as Criteria for Selection of Breeding Sows

3(80), vol. 2, 2014
UNC 631:15:658

There are results of the evaluation of reproductive traits of sows of two successive generations based on estimates of the indices KPVYA and SIVYAS. It was proved that selection based on performance data indexes does not provide the full animal’s selection of the best genotype, and does not involve the transfer of seed potential performance.