UDC 619:614. 31:637. 5:57. 083. 1
Formulation of the problem. A pressing problem of today is the effective control of poultry products for the availability of residual amounts of tetracycline group antibiotics, due to the fact that getting through the food chain to the human body, can cause overgrowth, toxemia, allergic reactions, secondary fungal infections, violation of mineral metabolism, is osteotropic and promote the development of antibiotic-resistant microorganisms in humans.
Statement of the problem. Now in Ukraine as part of periodic monitoring of poultry with the help of microbiological method only the residual amount of tetracycline was examined. However in September 2016 in connection with the entry into force of the Order. №695 from 08.06.2013, criteria of study of poultry products under the periodic control residual amounts of tetracycline antibiotics (oxytetracycline, doxycycline, tetracycline, hlorthlortetratsyklin) are expanded.
The purpose and objectives of research. Conducting of testing and validation of qualitative microbiological «NAT-screening» method of determination of tetracycline antibiotics in poultry, eggs and egg products.
Research methodology. The basis of this method is the principle of diffusion in agar, i.e. the ability of antibiotics to diffuse to the tight nutrient media inoculated by specific sensitive test organism causing inhybuvannya zone. This is reflected in the emergence of clearly defined agar, net growth of the test culture zones. Basically we took the microbiological screening method – «NAT-screening», which is used by the EU.
Results and discussion. The results are treated by methods of variation statistics using the program «Microsoft Excel – 15,0» from calculating the arithmetic mean (M), standard deviation (m) and the level of probability (p) by Student’s table. We determined the accuracy, specificity, sensitivity of method.
Conclusions and recommendations for further research. Established that sensitivity of European microbiological method «NAT-screening» of residual amounts of tetracycline antibiotics for meat was 50 mg / kg for eggs and egg products – 100 mg/kg, corresponding MDR European law and order №695 from 06.08.2013
Prospects for further research. Due to expansion of studying criteria of poultry products to residual amounts of antibiotics tetracycline group within the periodic monitoring should introduce the work of laboratories «NAT-screening».
Key words: test culture Bac.cereus ATCC 11778, tetracycline, oxytetracycline, chlortetracycline, doxycycline, poultry, eggs, NAT-screening, egg products.