UDC 612. 017. 1:616. 379-008. 64:611. 013:599. 323. 4
V. Kovpak, Doctor of veterinary sciences, senior lecturer
Y. Kharkevich, Doctor of veterinary sciences, senior lecturer
National University of Life and Environmental Sciences of Ukraine
Just as in humans, the pancreas of diabetic animals in unable to produce enough insulin due to damaged beta-islets. It was found that the total content of leukocytes in peripheral blood of diabetic animals and people correlates with the severity of impaired organs and systems of the body. Due to agrowing number of evidence that leukocytes play an important roles in the development of diabetic complications, greater attention in being given to the study of the subject.
The aim of this work in to investigate the level of glucose, total and differential leukocyte content in the peripheral blood of rats, some of their functional indexes,the content of circulating immune complexes and immunoglobulins against the background of thesimulatedalloxandiabetesbyintraperitonealadministrationofmesenchymalstemcells ofallogeneicorigin.
Materials and methods of research.We used 12 rats in the experiment. Rats were divided into 4 groups consisting of 3 rats each: I – control group, II – experimental group without therapeutic intervention (blood sampling for analysis was performed on the 20th day of the experiment), III – experimental group without therapeutic intervention (blood sampling for analysis was performed on the 34th day of the experiment), ІV – experimental group, where MSCs in an amount of 2 million were injected to animals on the 20th day after the formation of diabetes (blood sampling for analysis was performed on the 34th day of the experiment).
Alloxan diabetes was formed by a single subcutaneous injection of alloxan monohydrate in a dose of 150 mg/kg in the form of 5% solution on citrate buffer (pH 4.5) after a previous 24-hour starvation diet with free access to water.
MSCs wereobtainedfrombonemarrowoflongbonesofratsaged 7-10 days. Cellculture wasperformedbythestandardmethodinthe СО2incubator.
Experimentalfindings. The administration of alloxan to experimental animals causes the development of diabetes, accompanied by increased levels of glucose in the blood. The immunological blood parameters and functional parameters of phagocytes in diabetic animals indicate the presence of certain pathological processes caused by insulin deficiency in their body. Mesenchymal stem cells administered against the background of diabetes reduce the level of glucose in the blood of animals-recipients and help restore blood parameters toward the original indicators.
Key words: diabetes, mesenchymalstemcells, glucose, leukocytes, immunoglobulins.