Duda Yu., Prus M. Proteinogram and indicators of immunity during рassalurosis of rabbits with different level of invasion intensity

UDC 619:616.993.192.1:636.92

 

Yu. Duda

M. Prus

 

The purpose of our work was to determine the influence Passlurus ambiguus on proteinogramand indicators of cellular and humor alimmunity of rabbits.
Materials and methods. Analog groups of male rabbits of 3-5 months of age were selected for the experiments. Intensity of invasion was determined by the method of the MacMaster. By spectrophotometric method the blood of animals was determined: the content of total protein, albumin, globulin fractions, the level of IgA, IgG, IgM – discretede position method according to M. Kostina. The count of T- and B-lymphocytes was determined by the method of spontaneous rosette-formation with sheepery throcytes.
Results of the work. Rabbits with pasalurosis have different levels of invasion intensity (II): low (II = 276.47 ± 43.33 eggs in 1 g offeces), high (II = 2446.67 ± 422.11 eggs in 1 g offeces) – II and medium (II = 1293.75 ± 275.80 eggs in 1 g offeces) – III research groups. We did not find eggs in the control group. The total protein content was significantly (p<0.001) high from 1.38 times to 1.66 times compared with healthy ones, due to an increase in the content of globulins from 2.08 times (p<0.001) to 2.26 times (p<0.001 ), which led to a decrease in the protein ratio from 2.16 times (p<0.001) to 3.29 times (p<0.001) in sick animals with different II. Were corded a high content of γ-globulins almost 1.4 times in these animals compared to healthy ones. We observed a high content of β-globulins and α2-globulins by 6.70% and 7.05% (p<0.01) and 5.15% and 3.99% (p<0.05) in animals II and III experimental groups in comparison with control group. A decreased level of uricacid from 4.77 times (p <0.001) to 6.19 times (p<0.001) in rabbits with passalurosis is probably due to a violation of the process of its formation in the liver against the background of an increase in acid out put through the intestines and kidneys. The creatinine level in experimental rabbits was significantly higher in groups I,II,III by 42.64% (p<0.001), 46.49% (p<0.001) and 44.42% (p<0.001), respectively, compared with the control.
IgA and IgG levels were high (p<0.001) in comparison to healhty rabbits: 2.22 and 2.16 times (in animals with low II), 1.51 and 1.85 times (in animals with high II). We observed a significant (p<0.001) high level of IgM and В-lymphocytes againstthecontrol 1.58 times, 1.82 times, 1.70 timesandby11.19%, 15.20%, 13.07%,respectively, ingroups I, II, III of infected rabbits.
Conclusions. The content of total protein, globulins, γ-globulins, creatinine, IgA, IgG, IgM and В-lymphocytes were significantly higher (p<0.001) in the blood of sick rabbits than healthy ones. We observed significant changes in the proteinogram of rabbits with high levels of II. These changes indicate an increase in the body’s immune defense under the influence of Passalurus ambiguus. We found a decreased level of uricacid and a protein coefficient due to the low percentage of albuminin sick rabbits. This is possibly due to a violation of the process of their formation in the liver against the background of increased output.

Keywords: рassalurosis, protein metabolism, Passalurus ambiguus, albumin, globulin, globulin fractions, IgA, IgG, IgM,T, B-, O-lymphocytes.

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