{"id":4237,"date":"2018-10-30T11:10:54","date_gmt":"2018-10-30T09:10:54","guid":{"rendered":"https:\/\/visnyk.mnau.edu.ua\/?p=4237"},"modified":"2018-12-13T14:12:41","modified_gmt":"2018-12-13T12:12:41","slug":"n99v3r2018rokotyanska","status":"publish","type":"post","link":"https:\/\/visnyk.mnau.edu.ua\/en\/n99v3r2018rokotyanska\/","title":{"rendered":"V. O. Rokotianska. Influence of nanoclayates on the biological completeness of spermatozoids"},"content":{"rendered":"UDC 636.4:612.8<\/strong><\/span><\/p>\n

DOI<\/strong>: 10.31521\/2313-092X\/2018-3(99)-9<\/p>\n

V. O. Rokotianska<\/strong><\/p>\n

The experimental data on the effect of ferrous lactate on the qualitative parameters of sperm and the formation of prooxidine-antioxidant homeostasis in the semen of boars is represented.<\/em><\/p>\n

Experiments were conducted using adult boars of Ukrainian Large White pig breed; they were analogues by age, live weight and the quality of sperm production.<\/em><\/p>\n

In the experiment, semen samples were divided into three groups. I-group – control. In the experimental groups, solutions contained – 0.15 mg (group II) and 0.3 mg (group III) of ferrous lactate were added. The studied samples were incubated at 38 \u00b0 C for 12 and 24 hours.<\/em><\/p>\n

In the studied samples, the mobility and survival rate of sperm, the number of alive and dead sperm, and also the state of prooxidant-antioxidant homeostasis were determined.<\/em><\/p>\n

The process of incubating whole sperm for 24 hours leads to a significant reduction in sperm motility by 9% and survival of 9.8%. These reductions in the functional activity of sperm are due to the displacement of prooxidant-antioxidant homeostasis in the direction of accelerating the processes of peroxide oxidation.<\/em><\/p>\n

Adding 0.15 mg of ferrous lactate to whole sperm samples contributes to a 7% increase in survival of sperm during 12 hours of incubation with a subsequent decrease to 24 hours of incubation. These changes occur against the background of increased activity of catalase by 31,6% and superoxide dismutase 32,3% (p <0,05). Adding ferrous lactate for incubation in the amount of 0,3 mg stimulates the processes of peroxide oxidation – an increase in the concentration of DK 59% (p <0.01) and TBC 20.7% and a decrease in the activity of SOD at 8.06%, which leads to an increase functional activity of sperm. Prolongation of incubation for up to 24 hours leads to a decrease in the number of low molecular weight antioxidants: glutathione 62.3% (p <0.01) and ascorbic acid – 78% (p <0.01) and growth of dehydroascorbic acid at 135.78% (p <0 , 01), which is accompanied by a decrease in mobility of 18,2% (p <0,05) and survival of sperm 35,6% (p <0,01).<\/em><\/p>\n

Key words<\/em><\/strong>: sperm, boars, sperm production, peroxidation, TBA-active complexes, iron lactate.<\/em><\/p>\n

References:<\/strong><\/p>\n

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    UDC 636.4:612.8 DOI: 10.31521\/2313-092X\/2018-3(99)-9 V. O. Rokotianska The experimental data on the effect of ferrous lactate on the qualitative parameters of sperm and the formation of prooxidine-antioxidant homeostasis in the semen of boars is represented. Experiments were conducted using adult boars of Ukrainian Large White pig breed; they were analogues by age, live weight and […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[6,890],"tags":[912],"aioseo_notices":[],"views":418,"_links":{"self":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/posts\/4237"}],"collection":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/comments?post=4237"}],"version-history":[{"count":0,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/posts\/4237\/revisions"}],"wp:attachment":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/media?parent=4237"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/categories?post=4237"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/tags?post=4237"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}