Bechtold D. In Planta Agrobacterium Mediated Gene Transfer by Infiltration of Adult Arabidopsis thaliana Plants \/ D. Bechtold, J. Ellis, G. Pelletier \/\/ C. R. Acad. Sci., Life Sci. — 1993. — V. 316. — P. 1194—1199.<\/li>\n<\/ol>\nC. \u0412. \u0411\u043e\u0433\u0443\u043b\u044c\u0441\u043a\u0430\u044f. \u0410\u0433\u0440\u043e\u0431\u0430\u043a\u0442\u0435\u0440\u0438\u0430\u043b\u044c\u043d\u0430\u044f \u0442\u0440\u0430\u043d\u0441\u0444\u043e\u0440\u043c\u0430\u0446\u0438\u044f \u044f\u0440\u043e\u0432\u043e\u0433\u043e \u0440\u0430\u043f\u0441\u0430 \u0431\u0435\u0437 \u044d\u0442\u0430\u043f\u0430 \u0440\u0435\u0433\u0435\u043d\u0435\u0440\u0430\u0446\u0438\u0438 in vitro.<\/b>
\n\u0410\u043f\u0440\u043e\u0431\u0438\u0440\u043e\u0432\u0430\u043d\u0430 \u0442\u0440\u0430\u043d\u0441\u0444\u043e\u0440\u043c\u0430\u0446\u0438\u044f \u044f\u0440\u043e\u0432\u043e\u0433\u043e \u0440\u0430\u043f\u0441\u0430 \u0441 \u043f\u043e\u043c\u043e\u0449\u044c\u044e \u043f\u043e\u0447\u0432\u0435\u043d\u043d\u044b\u0445 \u0431\u0430\u043a\u0442\u0435\u0440\u0438\u0439 Agrobacterium tuimfaciens \u043c\u0435\u0442\u043e\u0434\u043e\u043c in planta. \u041f\u043e\u043a\u0430\u0437\u0430\u043d\u0430 \u0432\u043e\u0437\u043c\u043e\u0436\u043d\u043e\u0441\u0442\u044c \u043f\u043e\u043b\u0443\u0447\u0435\u043d\u0438\u044f \u0444\u043e\u0441\u0444\u0438\u043d\u043e\u0442\u0440\u0438\u0446\u0438\u043d-\u0440\u0435\u0437\u0438\u0441\u0442\u0435\u043d\u0442\u043d\u044b\u0445 \u0444\u043e\u0440\u043c \u044f\u0440\u043e\u0432\u043e\u0433\u043e \u0440\u0430\u043f\u0441\u0430, \u0430 \u0442\u0430\u043a\u0436\u0435 \u043f\u0435\u0440\u0435\u0434\u0430\u0447\u0438 \u043f\u0440\u0438\u0437\u043d\u0430\u043a\u0430 \u0443\u0441\u0442\u043e\u0439\u0447\u0438\u0432\u043e\u0441\u0442\u0438 \u0432 \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u0435 \u0441\u0430\u043c\u043e\u043e\u043f\u044b\u043b\u0435\u043d\u0438\u044f, \u043f\u0440\u0438 \u043a\u043e\u0442\u043e\u0440\u043e\u043c, \u0441\u043e\u0433\u043b\u0430\u0441\u043d\u043e \u043c\u0435\u043d\u0434\u0435\u043b\u0435\u0432\u0441\u043a\u0438\u043c \u0437\u0430\u043a\u043e\u043d\u043e\u043c\u0435\u0440\u043d\u043e\u0441\u0442\u044f\u043c, \u043f\u0440\u043e\u0438\u0437\u043e\u0448\u043b\u043e \u0440\u0430\u0441\u0449\u0435\u043f\u043b\u0435\u043d\u0438\u0435 3:1.<\/p>\n
\u0421\u0443\u0449\u0435\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u043c \u043f\u0440\u0435\u0438\u043c\u0443\u0449\u0435\u0441\u0442\u0432\u043e\u043c \u0434\u0430\u043d\u043d\u043e\u0433\u043e \u043c\u0435\u0442\u043e\u0434\u0430 \u044f\u0432\u043b\u044f\u0435\u0442\u0441\u044f \u043e\u0442\u0441\u0443\u0442\u0441\u0442\u0432\u0438\u0435 \u044d\u0442\u0430\u043f\u0430 \u0440\u0435\u0433\u0435\u043d\u0435\u0440\u0430\u0446\u0438\u0438 in vitr\u043e. \u0410 \u0442\u0430\u043a\u0436\u0435 \u0435\u0433\u043e \u043b\u0435\u0433\u043a\u043e\u0441\u0442\u044c \u0432 \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u043e\u0432\u0430\u043d\u0438\u0438 \u0438 \u043d\u0435\u0431\u043e\u043b\u044c\u0448\u0438\u0435 \u0444\u0438\u043d\u0430\u043d\u0441\u043e\u0432\u044b\u0435 \u0437\u0430\u0442\u0440\u0430\u0442\u044b.<\/p>\n
S. Bohulska. Agrobacterial transformation of spring rape without in vitro regeneration phase.<\/b>
\nThe objective of the research is testing the method of agrobacterium mediated transformation in planta for spring rape, and the possibility of obtaining herbicideresistant forms of the active substance phosphenotritsin.<\/p>\n
After inoculation into agrobacterium mediated suspension of spring rape flowers under the method in planta, we have obtained the seed that gave forms resistant to the Basta herbicide: 5 plants of Aidar variety, 4 plants of VNIS 100 variety, 9 plants of Dobrobut variety. As a result of self-pollination of these plants the seeds were harvested which was sown into the soil, and after young growth appearance they were sprayed with Basta. We obtained forms of spring rape that kept phosphenotritsin-resistance. This suggests that the bar gene implemented into the genome of plants, it is inherited and expressed.<\/p>\n","protected":false},"excerpt":{"rendered":"
\u041d\u043e\u043c\u0435\u0440, \u0440\u0456\u043a 2(78), 2014 \u0423\u0414\u041a 631.147:633.85 \u0410\u0432\u0442\u043e\u0440 C. \u0412. \u0411\u043e\u0433\u0443\u043b\u044c\u0441\u044c\u043a\u0430, \u0430\u0441\u043f\u0456\u0440\u0430\u043d\u0442 \u0423\u043c\u0430\u043d\u0441\u044c\u043a\u0438\u0439 \u043d\u0430\u0446\u0456\u043e\u043d\u0430\u043b\u044c\u043d\u0438\u0439 \u0443\u043d\u0456\u0432\u0435\u0440\u0441\u0438\u0442\u0435\u0442 \u0441\u0430\u0434\u0456\u0432\u043d\u0438\u0446\u0442\u0432\u0430 \u0410\u043d\u043e\u0442\u0430\u0446\u0456\u044f \u041e\u043f\u0442\u0438\u043c\u0456\u0437\u043e\u0432\u0430\u043d\u043e \u043f\u0440\u043e\u0446\u0435\u0441 \u0442\u0440\u0430\u043d\u0441\u0444\u043e\u0440\u043c\u0430\u0446\u0456\u0457 \u044f\u0440\u043e\u0433\u043e \u0440\u0456\u043f\u0430\u043a\u0443 \u0437\u0430 \u0434\u043e\u043f\u043e\u043c\u043e\u0433\u043e\u044e Agrobacterium tumefaciens \u043c\u0435\u0442\u043e\u0434\u043e\u043c in planta. \u041f\u0440\u043e\u0432\u0435\u0434\u0435\u043d\u043e \u043e\u0446\u0456\u043d\u043a\u0443 \u0440\u0435\u0437\u0438\u0441\u0442\u0435\u043d\u0442\u043d\u043e\u0441\u0442\u0456 \u043e\u0442\u0440\u0438\u043c\u0430\u043d\u0438\u0445 \u0444\u043e\u0440\u043c \u0440\u0456\u043f\u0430\u043a\u0443 \u0434\u043e \u0433\u0435\u0440\u0431\u0456\u0446\u0438\u0434\u0443 \u0456\u0437 \u0434\u0456\u044e\u0447\u043e\u044e \u0440\u0435\u0447\u043e\u0432\u0438\u043d\u043e\u044e \u0444\u043e\u0441\u0444\u0456\u043d\u043e\u0442\u0440\u0456\u0446\u0438\u043d. \u0412\u0456\u0434\u0456\u0431\u0440\u0430\u043d\u043e \u0444\u043e\u0440\u043c\u0438, \u044f\u043a\u0456 \u0443\u0441\u043f\u0430\u0434\u043a\u0443\u0432\u0430\u043b\u0438 \u0441\u0442\u0456\u0439\u043a\u0456\u0441\u0442\u044c \u0434\u043e \u0433\u0435\u0440\u0431\u0456\u0446\u0438\u0434\u0443. \u041a\u043b\u044e\u0447\u043e\u0432\u0456 \u0441\u043b\u043e\u0432\u0430 \u044f\u0440\u0438\u0439 \u0440\u0456\u043f\u0430\u043a, \u0430\u0433\u0440\u043e\u0431\u0430\u043a\u0442\u0435\u0440\u0456\u0457, \u0442\u0440\u0430\u043d\u0441\u0444\u043e\u0440\u043c\u0430\u0446\u0456\u044f, \u043c\u0435\u0442\u043e\u0434 in […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[346],"tags":[356],"aioseo_notices":[],"views":1157,"_links":{"self":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/posts\/738"}],"collection":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/comments?post=738"}],"version-history":[{"count":0,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/posts\/738\/revisions"}],"wp:attachment":[{"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/media?parent=738"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/categories?post=738"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/visnyk.mnau.edu.ua\/en\/wp-json\/wp\/v2\/tags?post=738"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}