R.O. Kulibaba, Y.V. Liashenko. Analysis of genetic differentiation of subpopulation of Ukrainian meat-egg purposed chickens using microsatellite markers

R.O. Kulibaba

Y.V. Liashenko

The study of the genetic structure of experimental populations of different species of animals and birds is one of the most actual problems of modern agricultural biology. Microsatellites are referred to one of the most effective tools for studying the genetic variability of populations.

The objective of the research is to determine the genetic differentiation of five subpopulations of Ukrainian dual-purpose chickens using microsatellite markers.

The research was carried out on five subpopulations of Ukrainian dual-purpose chickens – G-1, G-2, G-3, G-4 and C. The following microsatellite markers were used for the research: MCW0081, MCW0034, MCW0104, ADL0268, LEI0166, ADL0278, LEI0094, and MCW0123.

The avian feathers were used as a source of biological material. DNA from the test samples was isolated using a DNA-sorb-B commercial reagent kit (AmpliSens, Russia). The amplification was carried out using standard methods and appropriate reagents.

Polymorphism for each of studied microsatellite loci in the experimental subpopulations of dual-purpose chickens of Ukrainian selection was identified. The total indicators of genetic variability in all experimental groups differed. The total allele pool in all experimental populations for 8 selected microsatellite loci was presented by 38 alleles. The lowest genetic diversity for the number of alleles per locus in all experimental populations was shown for ADL0278 marker (3 alleles per locus), the largest – for MCW0104 (6.4 alleles per locus). According to the analysis of Write’s F-statistics, most of the revealed genetic variability corresponded to the intra-population component (9.2% of the total genetic variability were distributed between subpopulations and 90.8% within subpopulations). It was revealed that G-1 and G-4 subpopulations were the most remote by the values of genetics distances (28.8% of differences), while G-2 and G-3 subpopulations were the closest (13.3% of differences).

So, as results of the research, pronounced difference between subpopulations was revealed that corresponds to the average degree of divergence in experimental chicken groups. This reflects their common origin and the intensity of the breeding work being carried out.

Key words: population, chickens, microsatellites, polymorphism, allele, genetic structure.


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