UDC 575.827: 636.2.033
Genetic structure of the Southern meat cattle breed from the State Enterprise Experimental Farm “Askaniyske” NAAS (Kherson region) were investigated based on the microsatellite DNA loci. A total of 192 individuals were analyzed. Of those, 100 individuals represented “Santa Gertrudis” (SG) subpopulation and 92 ones represented “Zebu” (ZB) subpopulation. A panel of 4 bovine-speciﬁc microsatellite markers (TGLA53, TGLA122, TGLA126 and TGLA227), recommended of the ISAG for cattle genetic diversity studies, was selected for genetic characterization and revealing the extent of genetic diversity in the Southern Meat cattle breed.
Genomic DNA was extracted from tissue samples using Nexttec column (Nexttec Biotechnology GmbH, Germany) following the manufacturer’s instructions. The DNA concentration was estimated by measuring the absorbance at 260 nm and the DNA quality was checked by separation on agarose gels. PCR products were detected by АВI 3130xl (Applied Biosystems, USA), subsequently processed via GeneMapper ID v. 3.2 software. All laboratory tests were conducted in the laboratory of Molecular Genetics, Animal Center of Biotechnology and Molecular Diagnostics, All-Russian Research Institute for Animal Husbandry named after academy member L.K. Ernst.
For the SG-subpopulation, frequency of the TGLA53156, TGLA122143, TGLA22789 and TGLA22797 alleles is significantly higher, whereas frequency of the TGLA53162, TGLA53166, TGLA122145, TGLA122149 and TGLA22781 alleles is predominant for the ZB-subpopulation.
We report for the first time, the distribution and the frequency of a taurine and an indicine specific alleles amongst SG- and ZB-subpopulations of the Southern Meat cattle breed. Three alleles (TGLA122149, TGLA12612 and TGLA22777) were classified as zebu diagnostic; on other hand, three other alleles (TGLA53156, TGLA122143 and TGLA126115) were classified as taurine breeds diagnostic.
Key words: microsatellite DNA loci, genotypic and allelic richness, taurus/indicus-specific alleles, the Southern Meat cattle breed, cattle.